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t7-tag affinity purification kit  (Millipore)
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https://www.bioz.com/result/t7-tag affinity purification kit/product/Millipore
Average 90 stars, based on 1 article reviews
t7-tag affinity purification kit - by Bioz Stars, 2026-03
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t7.tag ® affinity purification kit  (Millipore)
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The <t>T7</t> antibody covalently linked agarose beads were incubated with the purified His-T7-tagged PpEPI10 (+ rPpEPI10) or buffer (- rPpEPI10). The mixture was added to buffer <t>(-</t> <t>HbSPA)</t> or the intercellular fluids isolated from N . benthamiana leaves expressing HbSPA-His protein (+ HbSPA). After washing, the eluates were analyzed by Western blot with HRP conjugated anti-His monoclonal antibody. Lane M represents the protein standard. The numbers on the left indicate the size of molecular weight markers.
T7.Tag ® Affinity Purification Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/t7.tag ® affinity purification kit/product/Millipore
Average 90 stars, based on 1 article reviews
t7.tag ® affinity purification kit - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
t7•tag affinity purification kit  (Millipore)
90
Millipore t7•tag affinity purification kit
The <t>T7</t> antibody covalently linked agarose beads were incubated with the purified His-T7-tagged PpEPI10 (+ rPpEPI10) or buffer (- rPpEPI10). The mixture was added to buffer <t>(-</t> <t>HbSPA)</t> or the intercellular fluids isolated from N . benthamiana leaves expressing HbSPA-His protein (+ HbSPA). After washing, the eluates were analyzed by Western blot with HRP conjugated anti-His monoclonal antibody. Lane M represents the protein standard. The numbers on the left indicate the size of molecular weight markers.
T7•Tag Affinity Purification Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/t7•tag affinity purification kit/product/Millipore
Average 90 stars, based on 1 article reviews
t7•tag affinity purification kit - by Bioz Stars, 2026-03
90/100 stars
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The T7 antibody covalently linked agarose beads were incubated with the purified His-T7-tagged PpEPI10 (+ rPpEPI10) or buffer (- rPpEPI10). The mixture was added to buffer (- HbSPA) or the intercellular fluids isolated from N . benthamiana leaves expressing HbSPA-His protein (+ HbSPA). After washing, the eluates were analyzed by Western blot with HRP conjugated anti-His monoclonal antibody. Lane M represents the protein standard. The numbers on the left indicate the size of molecular weight markers.

Journal: PLoS ONE

Article Title: The plant defense and pathogen counterdefense mediated by Hevea brasiliensis serine protease HbSPA and Phytophthora palmivora extracellular protease inhibitor PpEPI10

doi: 10.1371/journal.pone.0175795

Figure Lengend Snippet: The T7 antibody covalently linked agarose beads were incubated with the purified His-T7-tagged PpEPI10 (+ rPpEPI10) or buffer (- rPpEPI10). The mixture was added to buffer (- HbSPA) or the intercellular fluids isolated from N . benthamiana leaves expressing HbSPA-His protein (+ HbSPA). After washing, the eluates were analyzed by Western blot with HRP conjugated anti-His monoclonal antibody. Lane M represents the protein standard. The numbers on the left indicate the size of molecular weight markers.

Article Snippet: Co-immunoprecipitation (co-IP) was performed on the intercellular fluid containing the His-tagged HbSPA and the purified His-T7-tagged PpEPI10 (rPpEPI10) using the T7.tag ® Affinity Purification Kit (Novagen, Merck Millipore, MA, USA) following the manufacturer’s instructions, with minor modifications.

Techniques: Incubation, Purification, Isolation, Expressing, Western Blot, Molecular Weight